Multifunctional protein-enabled patterning on arrayed ferroelectric materials

ACS Appl Mater Interfaces. 2012 Apr;4(4):1865-71. doi: 10.1021/am300177t. Epub 2012 Apr 3.

Abstract

This study demonstrates a biological route to programming well-defined protein-inorganic interfaces with an arrayed geometry via modular peptide tag technology. To illustrate this concept, we designed a model multifunctional fusion protein, which simultaneously displays a maltose-binding protein (MBP), a green fluorescence protein (GFPuv) and an inorganic-binding peptide (AgBP2C). The fused combinatorially selected AgBP2C tag controls and site-directs the multifunctional fusion protein to immobilize on silver nanoparticle arrays that are fabricated on specific domain surfaces of ferroelectric LiNbO(3) via photochemical deposition and in situ synthesis. Our combined peptide-assisted biological and ferroelectric lithography approach offers modular design and versatility in tailoring surface reactivity for fabrication of nanoscale devices in environmentally benign conditions.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Kinetics
  • Maltose-Binding Proteins / chemistry*
  • Maltose-Binding Proteins / genetics
  • Maltose-Binding Proteins / metabolism
  • Nanoparticles / chemistry
  • Nanotechnology / instrumentation*
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / metabolism
  • Protein Array Analysis / instrumentation*
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Silver Nitrate / chemistry

Substances

  • Maltose-Binding Proteins
  • Peptides
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Silver Nitrate