Abstract
Protein kinases play an important role in regulating the response to abiotic stress in plant. CIPKs are plant-specific signal transducers, and some members have been identified. However, the precise functions of novel CIPKs still remain unknown. Here we report that HbCIPK2 is a positive regulator of salt and osmotic stress tolerance. HbCIPK2 was screened out of the differentially expressed fragments from halophyte Hordeum brevisubulatum by cDNA-AFLP technique, and was a single-copy gene without intron. Expression of HbCIPK2 was increased by salt, drought and ABA treatment. HbCIPK2 is mainly localized to the plasma membrane and nucleus. Ectopic expression of 35S:HbCIPK2 not only rescued the salt hypersensitivity in Arabidopsis mutant sos2-1, but also enhanced salt tolerance in Arabidopsis wild type, and exhibited tolerance to osmotic stress during germination. The HbCIPK2 contributed to the ability to prevent K(+) loss in root and to accumulate less Na(+) in shoot resulting in K(+) /Na(+) homeostasis and protection of root cell from death, which is consistent with the gene expression profile of HbCIPK2-overexpressing lines. These findings imply possible novel HbCIPK2-mediated salt signalling pathways or networks in H. brevisubulatum.
© 2012 Blackwell Publishing Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amplified Fragment Length Polymorphism Analysis
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Arabidopsis / genetics
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Arabidopsis / physiology
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Arabidopsis Proteins / metabolism
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Cytoprotection / drug effects
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DNA, Complementary / genetics
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Gene Expression Profiling
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Gene Expression Regulation, Plant / drug effects
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Hordeum / drug effects
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Hordeum / enzymology*
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Hordeum / genetics
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Hordeum / physiology*
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Molecular Sequence Data
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Mutation / genetics
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Osmotic Pressure / drug effects
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Phylogeny
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / cytology
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Plant Roots / drug effects
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Plant Roots / metabolism
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Potassium / metabolism
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Structure, Tertiary
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Protons
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Salt Tolerance / drug effects
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Salt Tolerance / genetics
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Salt Tolerance / physiology*
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Salt-Tolerant Plants / drug effects
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Salt-Tolerant Plants / enzymology*
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Salt-Tolerant Plants / genetics
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Salt-Tolerant Plants / physiology*
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Sequence Alignment
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Sodium / metabolism
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Sodium Chloride / pharmacology
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Stress, Physiological* / drug effects
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Stress, Physiological* / genetics
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Subcellular Fractions / drug effects
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Subcellular Fractions / enzymology
Substances
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Arabidopsis Proteins
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DNA, Complementary
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Plant Proteins
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Protons
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Sodium Chloride
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Sodium
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SOS2 protein, Arabidopsis
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Protein Serine-Threonine Kinases
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Potassium