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. 2012 May;19(5):659-65.
doi: 10.1128/CVI.00070-12. Epub 2012 Mar 29.

Analysis of the Bactericidal Response to an Experimental Neisseria Meningitidis Vesicle Vaccine

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Free PMC article

Analysis of the Bactericidal Response to an Experimental Neisseria Meningitidis Vesicle Vaccine

Elizabeth E Moran et al. Clin Vaccine Immunol. .
Free PMC article

Erratum in

  • Clin Vaccine Immunol. 2012 Oct;19(10)1705

Abstract

Rabbit immunogenicity studies on an experimental trivalent native outer membrane vesicle vaccine derived from three serogroup B strains were conducted to evaluate the effectiveness of this vaccine at inducing an antibody response with serum bactericidal activity against meningococcal strains of other serogroups in addition to serogroup B strains. The results showed that the vaccine was capable of inducing an effective broad-based bactericidal antibody response in rabbits against a small sample of Neisseria meningitidis strains of serogroups C, W135, and X and, to a lesser extent, serogroups A and Y. Analysis of antibody specificity using a bactericidal depletion assay revealed that antibodies to lipooligosaccharide (LOS), PorA, and NadA induced in rabbits by the experimental trivalent outer membrane vesicle vaccine were responsible for most of the bactericidal activity against strains of the other N. meningitidis serogroups. In the case of serogroup A N. meningitidis strains, the outer membrane antigen NadA was primarily responsible for protection. The outer membrane antigens fHbp and OpcA were also effective in removing some bactericidal activity from the sera.

Figures

Fig 1
Fig 1
Sera from the four rabbits immunized with the experimental trivalent vesicle vaccine were tested in the bactericidal assay against 4 serogroup A target strains (a). Pooled rabbit serum was analyzed for bactericidal antibody specificity using N. meningitidis serogroup A strain 8991 as the target strain in the bactericidal depletion assay (b).
Fig 2
Fig 2
Sera from the four rabbits immunized with the experimental trivalent vesicle vaccine were tested in the bactericidal assay (a) against 4 serogroup C target strains. Pooled rabbit serum was analyzed for bactericidal antibody specificity using N. meningitidis serogroup C strain 5660 as the target strain in the bactericidal depletion assay (b).
Fig 3
Fig 3
Sera from the four rabbits immunized with the experimental trivalent vesicle vaccine were tested in the bactericidal assay (a) against three different serogroup W135 target strains. Pooled rabbit serum was analyzed for bactericidal antibody specificity using N. meningitidis serogroup W135 strain 8122 as the target strain in the bactericidal depletion assay (b).
Fig 4
Fig 4
Sera from the four rabbits immunized with the experimental trivalent vesicle vaccine were tested in the bactericidal assay against three serogroup Y target strains (a). Pooled rabbit serum was analyzed for bactericidal antibody specificity using N. meningitidis serogroup Y strain 6972 as the target strain in the bactericidal depletion assay (b).
Fig 5
Fig 5
Sera from the four rabbits immunized with the experimental trivalent vesicle vaccine were tested in the bactericidal assay against three serogroup X test strains (a). Pooled rabbit serum was analyzed for bactericidal antibody specificity using N. meningitidis serogroup X strain 9557 as the target strain in the bactericidal depletion assay (b).

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