High-throughput species identification of enterococci using pyrosequencing

J Microbiol Methods. 2012 Jun;89(3):174-8. doi: 10.1016/j.mimet.2012.03.012. Epub 2012 Mar 23.

Abstract

Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Automation / methods
  • Bacterial Proteins / genetics*
  • Bacteriological Techniques / methods*
  • Chaperonins / genetics*
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics*
  • Enterococcus / classification*
  • Enterococcus / genetics*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Molecular Sequence Data

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • GroESL protein, Bacteria
  • Chaperonins

Associated data

  • GENBANK/JQ437400
  • GENBANK/JQ437401
  • GENBANK/JQ437402
  • GENBANK/JQ437403