Abstract
Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.
Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Animals
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Automation / methods
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Bacterial Proteins / genetics*
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Bacteriological Techniques / methods*
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Chaperonins / genetics*
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DNA, Bacterial / chemistry*
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DNA, Bacterial / genetics*
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Enterococcus / classification*
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Enterococcus / genetics*
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High-Throughput Nucleotide Sequencing / methods*
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Humans
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Molecular Sequence Data
Substances
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Bacterial Proteins
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DNA, Bacterial
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GroESL protein, Bacteria
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Chaperonins
Associated data
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GENBANK/JQ437400
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GENBANK/JQ437401
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GENBANK/JQ437402
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GENBANK/JQ437403