Hydroxysafflor yellow A alleviates early inflammatory response of bleomycin-induced mice lung injury

Biol Pharm Bull. 2012;35(4):515-22. doi: 10.1248/bpb.35.515.


Hydroxysafflor yellow A (HSYA) is an effective ingredient of Chinese herb Carthamus tinctorius L. The aim of this study was to evaluate the protective effect of HSYA on inflammatory phase of bleomycin-induced pulmonary injury in mice. Three doses of HSYA (26.7, 40, 60 mg/kg/d) were intraperitoneally injected to mice consecutively for 1 week after bleomycin administration. It was found that HSYA attenuated the loss in body weight, the increase of myeloperoxidase activity and pathologic changes of pulmonary inflammation caused by bleomycin. Treatment with HSYA also alleviated bleomycin-induced increase of mRNA level of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and transforming growth factor (TGF)-β1 in lung homogenates. Moreover HSYA inhibited the increased activation of nuclear factor (NF)-κB and phosphorylation of p38 mitogen-activated protein kinases (MAPK) in lung tissue. These findings demonstrated that HSYA had protective effect on bleomycin-induced lung inflammatory response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / drug therapy*
  • Acute Lung Injury / metabolism
  • Acute Lung Injury / pathology
  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Bleomycin
  • Carthamus
  • Chalcone / analogs & derivatives*
  • Chalcone / therapeutic use
  • Cytokines / genetics
  • Disease Models, Animal
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Peroxidase / metabolism
  • Phytotherapy
  • Plant Extracts
  • Quinones / therapeutic use*
  • RNA, Messenger / metabolism
  • Transcription Factor RelA / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism


  • Anti-Inflammatory Agents
  • Cytokines
  • Plant Extracts
  • Quinones
  • RNA, Messenger
  • Transcription Factor RelA
  • Bleomycin
  • hydroxysafflor yellow A
  • Chalcone
  • Peroxidase
  • p38 Mitogen-Activated Protein Kinases