Plant proteins, minerals and trace elements of Eurycoma longifolia (Tongkat Ali)

Nat Prod Res. 2013 Mar;27(4-5):314-8. doi: 10.1080/14786419.2012.676552. Epub 2012 Apr 3.


A water extraction method has been used to extract plant proteins from the roots of Eurycoma longifolia harvested from Perak and Pahang, Malaysia. On the basis of the spectroscopic Bradford assay, Tongkat Ali Perak and Pahang contained 0.3868 and 0.9573 mg mL(-1) of crude protein, respectively. The crude proteins were separated by one dimensional 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis into two (49.8 and 5.5 kD) and four (49.8, 24.7, 21.1 and 5.5 kD) protein spots for Tongkat Ali Perak and Pahang, respectively. Isoleucine was present in the highest concentration significantly. Both plant samples showed differences in the mineral and trace element profiles, but the minerals calcium, magnesium and potassium were present in the highest concentration. The highly concerned toxic metals such as arsenic and lead were not detected.

MeSH terms

  • Calcium / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Eurycoma / chemistry*
  • Magnesium / analysis
  • Minerals / analysis*
  • Plant Proteins / analysis*
  • Potassium / analysis
  • Trace Elements / analysis*


  • Minerals
  • Plant Proteins
  • Trace Elements
  • Magnesium
  • Potassium
  • Calcium