Jaceosidin induces p53-dependent G2/M phase arrest in U87 glioblastoma cells

Asian Pac J Cancer Prev. 2011;12(12):3235-8.

Abstract

Flavonoid compounds have been shown to trigger cell cycle arrest at G0/G1, S and G2/M checkpoints, allowing cells to repair DNA damage before entry into mitosis. Jaceosidin, a flavonoid compound, has been reported to induce apoptosis in various cancer cell lines. In our previous study, we established that jaceosidin induces apoptosis in U87 glioblastoma cells through G2/M phase arrest. However the molecular mechanisms oremained unclear. In the present study, mRNA and protein expression levels of major cell cycle regulatory genes were analyzed by semi-quantitative RT-PCR and Western blot studies respectively. The results demonstrated that jaceosidin-induced G2/M phase arrest in U87 cells is associated with DNA fragmentation, up-regulation of p53 and p21 and subsequent down-regulation of cyclin B1 and CDK1 expression at mRNA as well as at protein level. These findings provide insights into jaceosidin-induced G2/M phase arrest in U87 glioblastoma cells.

MeSH terms

  • Apoptosis / drug effects
  • Blotting, Western
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • CDC2 Protein Kinase / genetics
  • CDC2 Protein Kinase / metabolism
  • Cell Division / drug effects*
  • Cell Proliferation / drug effects
  • Cyclin B1 / genetics
  • Cyclin B1 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p21 / genetics
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Flavonoids / pharmacology*
  • G2 Phase / drug effects*
  • Glioblastoma / genetics
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology
  • Humans
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • CCNB1 protein, human
  • CDKN1A protein, human
  • Cyclin B1
  • Cyclin-Dependent Kinase Inhibitor p21
  • Flavonoids
  • RNA, Messenger
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • jaceosidin
  • CDC2 Protein Kinase