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, 2012, 829650

Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells

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Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells

Eun Jung Kwak et al. Mediators Inflamm.

Abstract

Objectives: In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells.

Methods: Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system.

Results: Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P < 0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P < 0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator.

Conclusion: Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.

Figures

Figure 1
Figure 1
Effect of magnolol on the growth of MC3T3-E1 cells. Data were expressed as a percentage of control. *P < 0.05 compared with control.
Figure 2
Figure 2
Effect of magnolol on the collagen synthesis of MC3T3-E1 cells. Data were expressed as a percentage of control. The control value for collagen content was 23.45 ± 0.659 μg/106 cells. *P < 0.05 compared with control.
Figure 3
Figure 3
Effect of magnolol on the alkaline phosphatase activity of MC3T3-E1 cells. Data were expressed as a percentage of control. The control value for ALP activity was 0.871 ± 0.016 Unit/mg. *P < 0.05 compared with control.
Figure 4
Figure 4
Effect of magnolol on the mineralization of osteoblastic MC3T3-E1 cells. Data were expressed as a percentage of control. The control value for mineralization was 0.504 ± 0.006 OD/106 cells. *P < 0.05 compared with control.
Figure 5
Figure 5
Effect of magnolol on the glutathione content of MC3T3-E1 cells. Data were expressed as a percentage of control. The control value for collagen content was 12.8 ± 0.305 nmole/mg. *P < 0.05 compared with control.
Figure 6
Figure 6
Effect of magnolol on antimycin A-induced RANKL production of MC3T3-E1 cells. Effect of magnolol on the production of RANKL in the presence of antimycin A. Osteoblasts were pre-incubated with magnolol before treatment with 70 μM antimycin A for 48 h. Data were expressed as a percentage of control. The control value for RANKL was 3.378 ± 0.074 ng/mg. *P < 0.05, control versus antimycin A; # P < 0.05, antimycin A versus magnolol.
Figure 7
Figure 7
Effect of magnolol on antimycin A-induced cytokines production of MC3T3-E1 cells. Osteoblasts were preincubated with magnolol before treatment with 70 μM antimycin A for 48 h. Data were expressed as a percentage of control. The control values for TNF-α (a) and IL-6 (b) were 0.228 ± 0.006 ng/mg and 0.655 ± 0.033 ng/mg, respectively. *P < 0.05, control versus antimycin A; # P < 0.05, antimycin A versus magnolol.

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References

    1. Karsenty G, Wagner EF. Reaching a genetic and molecular understanding of skeletal development. Developmental Cell. 2002;2(4):389–406. - PubMed
    1. Manolagas SC. The role of IL-6 type cytokines and their receptors in bone a. Annals of the New York Academy of Sciences. 1998;840:194–204. - PubMed
    1. Teitelbaum SL. Bone resorption by osteoclasts. Science. 2000;289(5484):1504–1508. - PubMed
    1. Wang JP, Hsu MF, Raung SL, Chen CC, Kuo JS, Teng CM. Anti-inflammatory and analgesic effects of magnolol. Naunyn-Schmiedeberg’s Archives of Pharmacology. 1992;346(6):707–712. - PubMed
    1. Teng CM, Yu SM, Chen CC, Huang YL, Huang TF. EDRF-release and Ca2+-channel blockade by magnolol, an antiplatelet agent isolated from Chinese herb Magnolia officinalis, in rat thoracic aorta. Life Sciences. 1990;47(13):1153–1161. - PubMed

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