scFv antibody: principles and clinical application

Clin Dev Immunol. 2012;2012:980250. doi: 10.1155/2012/980250. Epub 2012 Mar 15.

Abstract

To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes. This paper provides an overview of the current studies on the principle, generation, and application of scFv. The potential of scFv in breast cancer research is also discussed in this paper.

Publication types

  • Review

MeSH terms

  • Antibodies, Bispecific / biosynthesis
  • Antibodies, Bispecific / immunology
  • Antibodies, Bispecific / therapeutic use*
  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / immunology
  • Breast Neoplasms / therapy*
  • Escherichia coli
  • Female
  • Genes, Reporter
  • Humans
  • Immunotoxins / immunology
  • Immunotoxins / therapeutic use*
  • Molecular Targeted Therapy
  • Peptide Library
  • Plasmids
  • Protein Engineering
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / therapeutic use
  • Single-Chain Antibodies* / immunology
  • Single-Chain Antibodies* / therapeutic use
  • Transformation, Bacterial

Substances

  • Antibodies, Bispecific
  • Immunotoxins
  • Peptide Library
  • Recombinant Fusion Proteins
  • Single-Chain Antibodies