Selective chemical imaging of static actin in live cells

J Am Chem Soc. 2012 May 23;134(20):8480-6. doi: 10.1021/ja211708z. Epub 2012 May 14.


We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / analysis
  • Actins / ultrastructure*
  • Amanita / chemistry*
  • Bacterial Proteins / chemistry*
  • Biological Products / chemistry*
  • Cell Line
  • Cell Survival
  • Depsipeptides / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Microscopy, Fluorescence
  • Staining and Labeling / methods


  • Actins
  • Bacterial Proteins
  • Biological Products
  • Depsipeptides
  • Fluorescent Dyes
  • chondramide C
  • jasplakinolide