Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival

Microb Pathog. 2012 Jun;52(6):367-74. doi: 10.1016/j.micpath.2012.03.007. Epub 2012 Mar 29.


We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, macropinocytosis, and spacious phagosome formation. These events were associated with the rearrangement of actin and microtubule networks. The Salmonella pathogenesis island 1 (SPI-1) was necessary to invade B cells. In contrast to macrophages, B cells were highly resistant to cell death induced by Salmonella. These data demonstrate the ability of Salmonella to infect these non-professional phagocytic cells, where the bacterium can find an ideal intracellular niche to support persistence and the possible dissemination of infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • B-Lymphocytes / microbiology*
  • B-Lymphocytes / physiology*
  • Bacterial Proteins
  • Cells, Cultured
  • Female
  • Host-Pathogen Interactions*
  • Macrophages / microbiology
  • Macrophages / physiology
  • Mice
  • Mice, Inbred BALB C
  • Microtubules / metabolism
  • Phagosomes / microbiology*
  • Pinocytosis*
  • Salmonella / genetics
  • Salmonella / pathogenicity*
  • Virulence Factors / genetics
  • Virulence Factors / metabolism


  • Actins
  • Bacterial Proteins
  • Spi1 protein, Salmonella
  • Virulence Factors