Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation

Cell Immunol. Jan-Feb 2012;275(1-2):61-8. doi: 10.1016/j.cellimm.2012.02.016. Epub 2012 Mar 14.

Abstract

Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation*
  • Biomarkers / metabolism
  • Cell Differentiation
  • Cell Line
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Gene Expression Regulation*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / immunology*
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Interleukin-12 / genetics
  • Interleukin-12 / immunology
  • Mice
  • Transcription Factors / genetics
  • Transcription Factors / immunology*

Substances

  • Biomarkers
  • Hlx protein, mouse
  • Homeodomain Proteins
  • Transcription Factors
  • Interleukin-12
  • Interferon-gamma