Sensory regulation of the C. elegans germline through TGF-β-dependent signaling in the niche

Curr Biol. 2012 Apr 24;22(8):712-9. doi: 10.1016/j.cub.2012.02.064. Epub 2012 Apr 5.


The proliferation/differentiation balance of stem and progenitor cell populations must respond to the physiological needs of the organism [1, 2]. Mechanisms underlying this plasticity are not well understood. The C. elegans germline provides a tractable system to study the influence of the environment on progenitor cells (stem cells and their proliferative progeny). Germline progenitors accumulate during larval stages to form an adult pool from which gametes are produced. Notch pathway signaling from the distal tip cell (DTC) niche to the germline maintains the progenitor pool [3-5], and the larval germline cell cycle is boosted by insulin/IGF-like receptor signaling [6]. Here we show that, independent of its role in the dauer decision, TGF-β regulates the balance of proliferation versus differentiation in the C. elegans germline in response to sensory cues that report population density and food abundance. Ciliated ASI sensory neurons are required for TGF-β-mediated expansion of the larval germline progenitor pool, and the TGF-β receptor pathway acts in the germline stem cell niche. TGF-β signaling thereby couples germline development to the quality of the environment, providing a novel cellular and molecular mechanism linking sensory experience of the environment to reproduction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Caenorhabditis elegans Proteins / physiology*
  • Cell Differentiation
  • Cell Proliferation
  • Germ Cells / metabolism
  • Larva
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Pheromones / physiology
  • Population Density
  • Receptors, Notch / genetics
  • Receptors, Notch / metabolism
  • Receptors, Transforming Growth Factor beta / metabolism
  • Sensory Receptor Cells / cytology
  • Sensory Receptor Cells / metabolism*
  • Signal Transduction
  • Smad Proteins / genetics
  • Smad Proteins / metabolism
  • Stem Cell Niche
  • Stem Cells / metabolism
  • Transforming Growth Factor beta / metabolism*


  • Caenorhabditis elegans Proteins
  • DAF-3 protein, C elegans
  • Glp-1 protein, C elegans
  • Membrane Glycoproteins
  • Pheromones
  • Receptors, Notch
  • Receptors, Transforming Growth Factor beta
  • Smad Proteins
  • Transforming Growth Factor beta