An ORS-ICP-MS method for monitoring trace levels of cobalt and chromium in whole blood samples from hip arthroplasty patients with metal-on-metal prostheses

Clin Biochem. 2012 Jul;45(10-11):806-10. doi: 10.1016/j.clinbiochem.2012.03.025. Epub 2012 Mar 30.

Abstract

Objective: To develop a rapid and reliable method, using an octopole reaction system (ORS) ICP-MS, capable of monitoring trace levels of Co and Cr in whole blood samples from hip arthroplasty patients with metal-on-metal prostheses.

Design and method: Whole blood is diluted 10-fold with an alkaline diluent and analyzed using an Agilent 7500 CE ORS-ICP-MS.

Results: Limit of quantification of 0.03 μg/L Co and 0.20 μg/L Cr in patient samples. <6% covariance obtained for quality control materials analyzed over 10 runs.

Conclusion: This method is capable of monitoring trace levels of Co and Cr in diluted whole blood samples with a vial to vial run time of approximately 2 min. Results are comparable to those obtained using high resolution (HR) ICP-MS with sample digestion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys / chemistry
  • Arthroplasty, Replacement / methods*
  • Chromium / blood*
  • Chromium / chemistry
  • Cobalt / blood*
  • Cobalt / chemistry
  • Hip Prosthesis*
  • Humans
  • Reproducibility of Results
  • Spectrum Analysis / instrumentation
  • Spectrum Analysis / methods*
  • Trace Elements / blood

Substances

  • Alloys
  • Trace Elements
  • Chromium
  • Cobalt