SDS-Polyacrylamide Gel Electrophoresis of Proteins

Joseph Sambrook; David W Russell

doi:10.1101/pdb.prot4540

SDS-Polyacrylamide Gel Electrophoresis of Proteins

CSH Protoc. 2006 Sep 1;2006(4):pdb.prot4540. doi: 10.1101/pdb.prot4540.

Authors

Joseph Sambrook, David W Russell

PMID: 22485921
DOI: 10.1101/pdb.prot4540

Abstract

INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide. By using markers of known molecular weight, the molecular weight of the polypeptide chain(s) can be estimated.