Effects of Calendula officinalis on human gingival fibroblasts

Homeopathy. 2012 Apr;101(2):92-8. doi: 10.1016/j.homp.2012.02.003.

Abstract

Background: Calendula officinalis is commonly called the marigold. It is a staple topical remedy in homeopathic medicine. It is rich in quercetin, carotenoids, lutein, lycopene, rutin, ubiquinone, xanthophylls, and other anti-oxidants. It has anti-inflammatory properties. Quercetin, one of the active components in Calendula, has been shown to inhibit recombinant human matrix metalloproteinase (MMP) activity and decrease the expression of tumor necrosis factor-α, interleukin-1β (IL), IL-6 and IL-8 in phorbol 12-myristate 13-acetate and calcium ionophore-stimulated human mast cells.

Objectives: To examine the effects of Calendula on human gingival fibroblast (HGF) mediated collagen degradation and MMP activity.

Material and methods: Lactate dehydrogenate assays were performed to determine the non-toxic concentrations of Calendula, doxycycline and quercetin. Cell-mediated collagen degradation assays were performed to examine the inhibitory effect on cell-mediated collagen degradation. Gelatin zymography was performed to examine their effects on MMP-2 activity. The experiments were repeated three times and ANOVA used for statistical analyses.

Results: Calendula at 2-3% completely inhibited the MMP-2 activity in the zymograms. Doxycycline inhibited HGF-mediated collagen degradation at 0.005, 0.01, 0.02 and 0.05%, and MMP-2 activity completely at 0.05%. Quercetin inhibited HGF-mediated collagen degradation at 0.005, 0.01 and 0.02%, and MMP-2 activity in a dose-dependent manner. Calendula inhibited HGF-mediated collagen degradation and MMP-2 activity more than the same correlated concentration of pure quercetin.

Conclusion: Calendula inhibits HGF-mediated collagen degradation and MMP-2 activity more than the corresponding concentration of quercetin. This may be attributed to additional components in Calendula other than quercetin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / pharmacology
  • Calendula / chemistry*
  • Cells, Cultured / metabolism
  • Collagen / metabolism
  • Doxycycline / therapeutic use
  • Fibroblasts / drug effects*
  • Gingiva / drug effects*
  • Humans
  • Matrix Metalloproteinase Inhibitors*
  • Matrix Metalloproteinases / metabolism
  • Periodontal Diseases / drug therapy
  • Phytotherapy
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Quercetin / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Matrix Metalloproteinase Inhibitors
  • Plant Extracts
  • Collagen
  • Quercetin
  • Matrix Metalloproteinases
  • Doxycycline