Effect of dietary supplementation with Suaeda maritima on blood physiology, innate immune response, and disease resistance in olive flounder against Miamiensis avidus

Exp Parasitol. 2012 Jun;131(2):195-203. doi: 10.1016/j.exppara.2012.03.024. Epub 2012 Apr 3.

Abstract

The effect of Suaeda maritima enriched diet on blood physiology, innate immune response, and disease resistance in olive flounder Paralichythys olivaceus against Miamiensis avidus on weeks 1, 2, and 4 was investigated. Feeding with any enriched diet and then challenging with M. avidus significantly increased white blood cells (WBC) on weeks 2 and 4; the red blood cells (RBC) significantly increased with 0.1% and 1.0% enriched diets on week 4. The hemoglobin (Hb) and hematocrit (Ht) levels significantly increased when fed with 0.1% and 1.0% supplementation diets on weeks 2 and 4. The mean corpuscular volume (MCV) did not significantly vary with any diet and time; however the mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) significantly increased with 0.1% and 1.0% supplementation diets on weeks 2 and 4. The leukocytes such as lymphocytes (Lym), monocytes (Mon), neutrophils (Neu) and biochemical parameters such as total protein (TP), glucose (GLU), and calcium (CAL) levels significantly increased in 0.1% and 1.0% supplementation diet fed groups on weeks 2 and 4. The serum lysozyme activity was significantly enhanced in 0.1% and 1.0% supplementation diet fed groups from weeks 1 to 4 when compared to the control (0% herbal extract enriched diet). The scuticocidal activity and respiratory burst activity were significantly enhanced when fish were fed with 0.1% and 1.0% supplementation diets from weeks 2 and 4. The protective effect in terms of cumulative mortality (50% and 40%) was low in groups on being fed with 0.1% and 1.0% supplemented diet. Therefore the present study suggested that 0.1% and 1.0% S. maritime-supplemented diets protect the hematological and biochemical parameters, improving the innate immunity, affording protection disease from M. avidus infection in olive flounder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Cell Count / veterinary
  • Blood Chemical Analysis / veterinary
  • Blood Physiological Phenomena
  • Chenopodiaceae* / chemistry
  • Ciliophora Infections / diet therapy
  • Ciliophora Infections / immunology
  • Ciliophora Infections / prevention & control
  • Ciliophora Infections / veterinary*
  • Diet / veterinary*
  • Dietary Supplements
  • Disease Resistance
  • Erythrocyte Indices / veterinary
  • Fish Diseases / diet therapy
  • Fish Diseases / immunology
  • Fish Diseases / parasitology
  • Fish Diseases / prevention & control*
  • Flounder / blood
  • Flounder / immunology
  • Flounder / parasitology*
  • Hematocrit / veterinary
  • Hemoglobins / analysis
  • Immunity, Innate
  • Muramidase / blood
  • Oligohymenophorea*
  • Plant Extracts / administration & dosage
  • Random Allocation
  • Superoxides / metabolism

Substances

  • Hemoglobins
  • Plant Extracts
  • Superoxides
  • Muramidase