Innate immunity gene single nucleotide polymorphisms and otitis media

Int J Pediatr Otorhinolaryngol. 2012 Jul;76(7):976-9. doi: 10.1016/j.ijporl.2012.03.011. Epub 2012 Apr 9.


Objective: Toll-like receptors (TLR) activate the innate immune system. Single nucleotide polymorphisms (SNPs) in TLR genes are linked to increased susceptibility to infections. TLR4-deficient mice have increased incidence and duration of otitis media. We hypothesize that SNPs in TLR genes are more common in otitis-prone children than in children without a history of otitis media.

Methods: Cases (n=70) included children undergoing surgery for otitis media. Control subjects (n=70) included children undergoing surgery for non-otologic indication. Genomic DNA was extracted from blood samples. RT-PCR genotyping was performed for TLR2 (rs5743708), TLR4 (rs4986790 and rs4986791), TLR9 (rs5743836 & rs187084), and CD14 (rs2569190).

Results: There were no significant differences between the groups in family history, day care, smoke exposure, allergies or prevalence of the SNPs. The most common pre-op diagnosis in control subjects was obstructive sleep apnea (OSA).

Conclusions: TLR2, TLR4, TLR9 and CD14 gene SNPs were not more prevalent in otitis-prone children.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Immunity, Innate / genetics*
  • Infant
  • Lipopolysaccharide Receptors / genetics*
  • Male
  • Otitis Media / genetics*
  • Otitis Media / immunology*
  • Polymorphism, Single Nucleotide
  • Toll-Like Receptors / genetics*


  • Lipopolysaccharide Receptors
  • Toll-Like Receptors