Microvesicles derived from endothelial progenitor cells protect the kidney from ischemia-reperfusion injury by microRNA-dependent reprogramming of resident renal cells

Kidney Int. 2012 Aug;82(4):412-27. doi: 10.1038/ki.2012.105. Epub 2012 Apr 11.


Endothelial progenitor cells are known to reverse acute kidney injury by paracrine mechanisms. We previously found that microvesicles released from these progenitor cells activate an angiogenic program in endothelial cells by horizontal mRNA transfer. Here, we tested whether these microvesicles prevent acute kidney injury in a rat model of ischemia-reperfusion injury. The RNA content of microvesicles was enriched in microRNAs (miRNAs) that modulate proliferation, angiogenesis, and apoptosis. After intravenous injection following ischemia-reperfusion, the microvesicles were localized within peritubular capillaries and tubular cells. This conferred functional and morphologic protection from acute kidney injury by enhanced tubular cell proliferation, reduced apoptosis, and leukocyte infiltration. Microvesicles also protected against progression of chronic kidney damage by inhibiting capillary rarefaction, glomerulosclerosis, and tubulointerstitial fibrosis. The renoprotective effect of microvesicles was lost after treatment with RNase, nonspecific miRNA depletion of microvesicles by Dicer knock-down in the progenitor cells, or depletion of pro-angiogenic miR-126 and miR-296 by transfection with specific miR-antagomirs. Thus, microvesicles derived from endothelial progenitor cells protect the kidney from ischemic acute injury by delivering their RNA content, the miRNA cargo of which contributes to reprogramming hypoxic resident renal cells to a regenerative program.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury / genetics
  • Acute Kidney Injury / metabolism
  • Acute Kidney Injury / pathology
  • Acute Kidney Injury / prevention & control*
  • Animals
  • Apoptosis
  • Capillaries / metabolism
  • Capillaries / pathology
  • Cell Hypoxia
  • Cell Proliferation
  • Cell-Derived Microparticles / metabolism
  • Cell-Derived Microparticles / pathology
  • Cell-Derived Microparticles / transplantation*
  • Cells, Cultured
  • Chemotaxis, Leukocyte
  • Disease Models, Animal
  • Endothelial Cells / metabolism
  • Endothelial Cells / pathology
  • Endothelial Cells / transplantation*
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Fibrosis
  • Gene Expression Regulation
  • Kidney / blood supply
  • Kidney / metabolism*
  • Kidney / pathology
  • Kidney Tubules / metabolism
  • Kidney Tubules / pathology
  • Male
  • MicroRNAs / metabolism*
  • Oligonucleotides / metabolism
  • RNA Interference
  • Rats
  • Rats, Wistar
  • Regeneration
  • Reperfusion Injury / genetics
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Reperfusion Injury / prevention & control*
  • Ribonuclease III / genetics
  • Ribonuclease III / metabolism
  • Stem Cell Transplantation*
  • Stem Cells* / metabolism
  • Stem Cells* / pathology
  • Time Factors
  • Transfection


  • MIRN126 microRNA, rat
  • MicroRNAs
  • Oligonucleotides
  • Ribonuclease III