Cell type-specific protein and transcription profiles implicate periarbuscular membrane synthesis as an important carbon sink in the mycorrhizal symbiosis

Plant Signal Behav. 2012 Apr;7(4):461-4. doi: 10.4161/psb.19650. Epub 2012 Apr 1.

Abstract

The development of an arbuscular mycorrhizal (AM) symbiosis is a non-synchronous process with typical mycorrhizal root containing different symbiotic stages at one time. Methods providing cell type-specific resolution are therefore required to separate these stages and analyze each particular structure independently from each other. We established an experimental system for analyzing specific proteomic changes in arbuscule-containing cells of Glomus intraradices colonized Medicago truncatula roots. The combination of laser capture microdissection (LCM) and liquid chromatography-tandem mass chromatography (LC-MS/MS) allowed the identification of proteins with specific or increased expression in arbuscule-containing cells. Consistent with previous transcriptome data, the proteome of arbuscule-containing cells showed an increased number of proteins involved in lipid metabolism, most likely related to the synthesis of the periarbuscular membrane. In addition, transcriptome data of non-colonized cells of mycorrhizal roots suggest mobilization of carbon resources and their symplastic transport toward arbuscule-containing cells for the synthesis of periarbuscular membranes. This highlights the periarbuscular membrane as important carbon sink in the mycorrhizal symbiosis.

MeSH terms

  • Biological Transport / genetics
  • Carbohydrate Metabolism / genetics
  • Carbon Sequestration / genetics*
  • Gene Expression Profiling*
  • Gene Expression Regulation, Plant
  • Glomeromycota / physiology
  • Laser Capture Microdissection
  • Lipid Metabolism / genetics
  • Medicago truncatula / cytology
  • Medicago truncatula / genetics
  • Medicago truncatula / microbiology*
  • Mycorrhizae / physiology*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Roots / cytology*
  • Plant Roots / genetics
  • Plant Roots / microbiology
  • Proteome / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Symbiosis / genetics*

Substances

  • Plant Proteins
  • Proteome
  • RNA, Messenger