Abstract
ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA responses. In this study, we show that ROPGEF1 and ROPGEF4 interact with ROP11 on plasma membranes in guard cells. Furthermore, our analyses of ROPGEF1/4 knockout mutants and overexpressing lines suggested that ROPGEF1 and ROPGEF4 are specific regulators of ROP11 function in ABA-mediated stomatal closure.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / metabolism*
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Arabidopsis / anatomy & histology
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Arabidopsis / cytology*
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Membrane / metabolism
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Droughts
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Gene Expression Regulation, Plant
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Plant Stomata / metabolism*
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Protein Transport
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Signal Transduction
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Stress, Physiological
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rho GTP-Binding Proteins / metabolism*
Substances
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Arabidopsis Proteins
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Guanine Nucleotide Exchange Factors
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ROPGEF4 protein, Arabidopsis
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RopGEF1 protein, Arabidopsis
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Abscisic Acid
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ROP11 protein, Arabidopsis
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rho GTP-Binding Proteins