ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-mediated stomatal closure in Arabidopsis

FEBS Lett. 2012 May 7;586(9):1253-8. doi: 10.1016/j.febslet.2012.03.040. Epub 2012 Mar 28.

Abstract

ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA responses. In this study, we show that ROPGEF1 and ROPGEF4 interact with ROP11 on plasma membranes in guard cells. Furthermore, our analyses of ROPGEF1/4 knockout mutants and overexpressing lines suggested that ROPGEF1 and ROPGEF4 are specific regulators of ROP11 function in ABA-mediated stomatal closure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / metabolism*
  • Arabidopsis / anatomy & histology
  • Arabidopsis / cytology*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Membrane / metabolism
  • Droughts
  • Gene Expression Regulation, Plant
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism*
  • Plant Stomata / metabolism*
  • Protein Transport
  • Signal Transduction
  • Stress, Physiological
  • rho GTP-Binding Proteins / metabolism*

Substances

  • Arabidopsis Proteins
  • Guanine Nucleotide Exchange Factors
  • ROPGEF4 protein, Arabidopsis
  • RopGEF1 protein, Arabidopsis
  • Abscisic Acid
  • ROP11 protein, Arabidopsis
  • rho GTP-Binding Proteins