Dom34:hbs1 plays a general role in quality-control systems by dissociation of a stalled ribosome at the 3' end of aberrant mRNA

Mol Cell. 2012 May 25;46(4):518-29. doi: 10.1016/j.molcel.2012.03.013. Epub 2012 Apr 11.

Abstract

Translation arrest leads to an endonucleolytic cleavage of mRNA that is termed no-go decay (NGD). It has been reported that the Dom34:Hbs1 complex stimulates this endonucleolytic cleavage of mRNA induced by translation arrest in vivo and dissociates subunits of a stalled ribosome in vitro. Here we report that Dom34:Hbs1 dissociates the subunits of a ribosome that is stalled at the 3' end of mRNA in vivo, and has a crucial role in both NGD and nonstop decay. Dom34:Hbs1-mediated dissociation of a ribosome that is stalled at the 3' end of mRNA is required for degradation of a 5'-NGD intermediate. Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates. We propose that Dom34:Hbs1 stimulates degradation of the 5'-NGD intermediate and of nonstop mRNA by dissociating the ribosome that is stalled at the 3' end of the mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry
  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism
  • Base Sequence
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism*
  • Endoribonucleases / chemistry
  • Endoribonucleases / genetics*
  • Endoribonucleases / metabolism*
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism*
  • HSP70 Heat-Shock Proteins / chemistry
  • HSP70 Heat-Shock Proteins / genetics*
  • HSP70 Heat-Shock Proteins / metabolism*
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / genetics
  • Multiprotein Complexes / metabolism
  • Nonsense Mediated mRNA Decay
  • Peptide Chain Elongation, Translational
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / genetics*
  • Peptide Elongation Factors / metabolism*
  • Protein Interaction Domains and Motifs
  • RNA Stability*
  • RNA, Fungal / genetics*
  • RNA, Fungal / metabolism*
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism*
  • Ribosomes / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Cell Cycle Proteins
  • HBS1 protein, S cerevisiae
  • HSP70 Heat-Shock Proteins
  • Multiprotein Complexes
  • Peptide Elongation Factors
  • RNA, Fungal
  • RNA, Messenger
  • SKI7 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Dom34 protein, S cerevisiae
  • Endoribonucleases
  • GTP-Binding Proteins