The function of mitochondrial F(O)F(1) ATP-synthase from the whiteleg shrimp Litopenaeus vannamei muscle during hypoxia

Comp Biochem Physiol B Biochem Mol Biol. 2012 Aug;162(4):107-12. doi: 10.1016/j.cbpb.2012.03.004. Epub 2012 Apr 5.

Abstract

The effect of hypoxia and re-oxygenation on the mitochondrial complex F(O)F(1)-ATP synthase was investigated in the whiteleg shrimp Litopenaeus vannamei. A 660 kDa protein complex isolated from mitochondria of the shrimp muscle was identified as the ATP synthase complex. After 10h at hypoxia (1.5-2.0 mg oxygen/L), the concentration of L-lactate in plasma increased significantly, but the ATP amount and the concentration of ATPβ protein remained unaffected. Nevertheless, an increase of 70% in the ATPase activity was detected, suggesting that the enzyme may be regulated at a post-translational level. Thus, during hypoxia shrimp are able to maintain ATP amounts probably by using some other energy sources as phosphoarginine when an acute lack of energy occurs. During re-oxygenation, the ATPase activity decreased significantly and the ATP production continued via the electron transport chain and oxidative phosphorylation. The results obtained showed that shrimp faces hypoxia partially by hydrolyzing the ATP through the reaction catalyzed by the mitochondrial ATPase which increases its activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Anaerobiosis
  • Animals
  • Arthropod Proteins / genetics
  • Arthropod Proteins / metabolism
  • Arthropod Proteins / physiology*
  • Cell Hypoxia
  • Gene Expression
  • Lactic Acid / blood
  • Mitochondria, Muscle / enzymology*
  • Mitochondrial Proton-Translocating ATPases / metabolism
  • Mitochondrial Proton-Translocating ATPases / physiology*
  • Muscles / cytology
  • Muscles / enzymology*
  • Muscles / physiology
  • Oxygen / blood
  • Penaeidae / metabolism*
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • Tail

Substances

  • Arthropod Proteins
  • Protein Subunits
  • Lactic Acid
  • Adenosine Triphosphate
  • Mitochondrial Proton-Translocating ATPases
  • Oxygen