Abstract
Objectives:
To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC).
Methods:
After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues.
Results:
LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated.
Conclusions:
Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antibodies, Neoplasm / blood*
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Antigens, Ly / genetics
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Antigens, Ly / immunology*
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Antigens, Ly / metabolism
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Antigens, Neoplasm / genetics
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Antigens, Neoplasm / immunology*
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Antigens, Neoplasm / metabolism
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Area Under Curve
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Biomarkers, Tumor / blood*
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Carcinoma, Squamous Cell / blood*
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Carcinoma, Squamous Cell / diagnosis
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Carcinoma, Squamous Cell / immunology
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Case-Control Studies
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Cloning, Molecular
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Esophageal Neoplasms / blood*
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Esophageal Neoplasms / diagnosis
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Esophageal Neoplasms / immunology
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Female
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GPI-Linked Proteins / genetics
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GPI-Linked Proteins / immunology
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GPI-Linked Proteins / metabolism
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Humans
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Male
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Middle Aged
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ROC Curve
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Transcription, Genetic
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Up-Regulation
Substances
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Antibodies, Neoplasm
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Antigens, Ly
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Antigens, Neoplasm
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Biomarkers, Tumor
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GPI-Linked Proteins
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LY6K protein, human