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. 2012 Apr 17;22(4):703-5.
doi: 10.1016/j.devcel.2012.03.008.

The tumor suppressor merlin controls growth in its open state, and phosphorylation converts it to a less-active more-closed state

Ifat Sher, C Oliver Hanemann, P Andrew Karplus, Anthony Bretscher

The tumor suppressor merlin controls growth in its open state, and phosphorylation converts it to a less-active more-closed state

Ifat Sher et al. Dev Cell. .
No abstract available

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Figures

Figure 1
Figure 1
A. GST-Cter fragments bound to glutathione-Sepharose beads were used to recover untagged Nter expressed in bacteria. B. Quantification of Nter binding (mean ± SD). C. 293 cells expressing HA-Nter and either vector (V) or Myc-tagged Cter (Myc-Cter). Myc immunoprecipitates were immunoblotted as indicated. D. Untagged Cter incubated with p21 activated kinase (PAK2) in the presence (+) or absence (−) of ATP, resolved on 15% SDS-PAGE gel and immunoblotted with anti-phospho-merlin (p-S518) (red) and anti-merlin (green) (Upper panel) or total merlin (lower panel). E. Immobilized Nter was used to recover phosphorylated WT Cter or mutated Cter fragments. F. Relative binding by GST-EBP50 to full-length mutants expressed in 293T cells (mean ± SD). G. Merlin levels in HEI-193 cells stably expressing merlin variants. H. HEI-193 cells were serum starved, restimulated with serum, and examined for relative BrdU incorporation (mean ± SD).
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