Promotion of Hair Growth by Rosmarinus Officinalis Leaf Extract

Phytother Res. 2013 Feb;27(2):212-7. doi: 10.1002/ptr.4712. Epub 2012 Apr 20.

Abstract

Topical administration of Rosmarinus officinalis leaf extract (RO-ext, 2 mg/day/mouse) improved hair regrowth in C57BL/6NCrSlc mice that experienced hair regrowth interruption induced by testosterone treatment. In addition, RO-ext promoted hair growth in C3H/He mice that had their dorsal areas shaved. To investigate the antiandrogenic activity mechanism of RO-ext, we focused on inhibition of testosterone 5α-reductase, which is well recognized as one of the most effective strategies for the treatment of androgenic alopecia. RO-ext showed inhibitory activity of 82.4% and 94.6% at 200 and 500 µg/mL, respectively. As an active constituent of 5α-reductase inhibition, 12-methoxycarnosic acid was identified with activity-guided fractionation. In addition, the extract of R. officinalis and 12-methoxycarnosic acid inhibited androgen-dependent proliferation of LNCaP cells as 64.5% and 66.7% at 5 µg/mL and 5 μM, respectively. These results suggest that they inhibit the binding of dihydrotestosterone to androgen receptors. Consequently, RO-ext is a promising crude drug for hair growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-alpha Reductase Inhibitors / pharmacology*
  • Alopecia / chemically induced
  • Alopecia / drug therapy
  • Androgen Antagonists / pharmacology
  • Animals
  • Cell Line, Tumor
  • Hair / drug effects*
  • Hair / growth & development
  • Humans
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Phytotherapy
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Rats
  • Rats, Wistar
  • Rosmarinus / chemistry*
  • Testosterone / pharmacology

Substances

  • 5-alpha Reductase Inhibitors
  • Androgen Antagonists
  • Plant Extracts
  • Testosterone