[Complete genome sequence characteristics of human enterovirus 71 strain isolated in Yunnan, China]

Bing Du Xue Bao. 2012 Mar;28(2):108-13.
[Article in Chinese]


The complete nucleotide sequence of two human enterovirus 71 strains (KMM09 and KM186-09) isolated in Yunnan,China, were determined by RT-PCR and sequencing. As with other human enteroviruses, the genomes were 7 409 nucleotides (nts) in length and encoded 2 193 aa. Phylogenetic analysis based on VP1 regions revealed that the two isolates belonged to subgenotype C4a. In structural genomic regions, subgenotype C4 was most homologous to other strains of C genotype when compared to other genotypes. In non-structural genomic regions, subgenotype C4 was more homologous to CA16/G10 and other strains of B genotype than to other strains of C genotype. RDP3 and Blast analysis displayed evidence of recombination in non-structural genomic regions between subgenotype B3 and C4, C4 and CA16/G10. The full-length genome of the human enterovirus 71 strains provided an overview of the diversity of genetic characteristics of a circulatinghuman enterovirus 71.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • China
  • Chlorocebus aethiops
  • Enterovirus A, Human / classification
  • Enterovirus A, Human / genetics*
  • Enterovirus A, Human / isolation & purification*
  • Feces / virology
  • Genome, Viral*
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Vero Cells

Associated data

  • GENBANK/HQ423142
  • GENBANK/HQ423143