2012 May 22
Phosphoregulation of Spc105 by Mps1 and PP1 Regulates Bub1 Localization to Kinetochores
Item in Clipboard
Phosphoregulation of Spc105 by Mps1 and PP1 Regulates Bub1 Localization to Kinetochores
Kinetochores are the macromolecular complexes that interact with microtubules to mediate chromosome segregation. Accurate segregation requires that kinetochores make bioriented attachments to microtubules from opposite poles. Attachments between kinetochores and microtubules are monitored by the spindle checkpoint, a surveillance system that prevents anaphase until every pair of chromosomes makes proper bioriented attachments. Checkpoint activity is correlated with the recruitment of checkpoint proteins to the kinetochore. Mps1 is a conserved protein kinase that regulates segregation and the spindle checkpoint, but few of the targets that mediate its functions have been identified. Here, we show that Mps1 is the major kinase activity that copurifies with budding yeast kinetochore particles and identify the conserved Spc105/KNL-1/blinkin kinetochore protein as a substrate. Phosphorylation of conserved MELT motifs within Spc105 recruits the Bub1 protein to kinetochores, and this is reversed by protein phosphatase I (PP1). Spc105 mutants lacking Mps1 phosphorylation sites are defective in the spindle checkpoint and exhibit growth defects. Together, these data identify Spc105 as a key target of the Mps1 kinase and show that the opposing activities of Mps1 and PP1 regulate the kinetochore localization of the Bub1 protein.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Figure 1. Mps1 kinase activity specifically co-purifies with native kinetochore complexes
(A) WT (SBY8253),
cdc28-13 (SBY8716), ipl1-321 (SBY8712) Ubi-R-TetR-BUB1 (SBY8920) and mps1-1 (SBY8726) strains were shifted to conditions to inactivate the kinases and kinetochores were purified by α-Flag immunoprecipitation of Dsn1-His-Flag. The kinetochores were treated with γ- 32P-ATP and eluted with 3×-Flag peptide. Particle composition was analyzed by silver-stained SDS-PAGE (left) and radioactive incorporation was analyzed by autoradiography (right). (B) Wild type and mps1-1 kinetochore complexes prepared as in (A) were analyzed by immunoblotting with the indicated antibodies. The relative amount of sample loaded in each lane is indicated at top.
Figure 2. The association of Bub1 with kinetochores is regulated by Mps1-mediated phosphorylation
pMet-Cdc20 BUB1-3GFP MTW1-mCherry cells containing MPS1 (SBY10318) or mps1-as1 (SBY10282) were arrested in mitosis by Cdc20 depletion and treated with nocodazole. They were then incubated with (+ inhibitor) or without (− inhibitor) 1NM-PP1 and analyzed for co-localization between Bub1 and Mtw1. Scale bar = 5.0 microns. (B) Kinetochore particles from strains containing Bub1-3GFP and MPS1 (SBY8502) or mps1-1 (SBY9348) were purified and analyzed by immunoblotting. (C) Kinetochore particles were purified from a strain containing Bub1-3GFP (SBY8502). The particles were treated with PP1 in the presence or absence of phosphatase inhibitors and the bound and unbound pools were analyzed by immunoblotting. (D) Kinetochore particles were purified from strains containing Bub1-3GFP and MPS1 (SBY8502) or mps1-1 (SBY9348). The particles were treated with PP1 and then incubated with +/− ATP. They were then incubated in a lysate from a WT strain (SBY8502) and analyzed by immunoblotting. (E) BUB1-3GFP NUF2-mCherry pGAL-MPS1 cells (SBY10271) were arrested in G1 and then treated with or without galactose. The co-localization of Bub1 with Nuf2 was analyzed by microscopy. Scale bar = 5.0 microns.
Figure 3. Mps1 and Glc7 regulate the Spc105/Bub1 interaction
(A) Kinetochore particles from WT (SBY8253),
SPC105-3GFP (SBY8750), mps1-1 (SBY8726), and SPC105-3GFP mps1-1 (SBY9099) strains were treated as in Fig 1A, as was a control purification from untagged strain SBY3. Recombinant GST-Mps1 was incubated with the mps1-1 samples. (B) Spc105-3Flag was immunoprecipitated from BUB1-13myc control cells (SBY3269) grown at 23 °C and from SPC105-3Flag (SBY7420), SPC105-3Flag BUB1-13myc (SBY9226) and SPC105-3Flag BUB1-13myc mps1-1 (SBY9476) cells shifted to 37 °C for 2 hours before harvest. Samples were analyzed by immunoblotting. (C) Spc105-3Flag was immunoprecipitated from WT (SBY9226), pGAL-MPS1 (SBY10100), and pGAL-GLC7 (SBY9971) cells containing BUB1-13myc and analyzed by immunoblotting. Control strains containing BUB1-13myc but lacking Spc105-3Flag are SBY4020 ( pGAL-GLC7), SBY3269 (WT) and SBY4587 ( pGAL-Mps1). (D) pMET-CDC20 cells containing Spc105-Flag and/or Bub1-13myc (SBY10464, SBY10364, SBY10465 and SBY10466) were arrested in metaphase and then galactose was added to induce Mps1 overexpression and cells were processed as in (C). (E) Spc105-3Flag was purified from cells arrested in G1 and treated with galactose for one hour to induce Mps1 overexpression. The strains used were BUB1-13myc (SBY3269), BUB1-13myc pGAL-MPS1 (SBY4587), SPC105-3Flag (SBY7492), SPC105-3Flag pGal-MPS1 (SBY9952), SPC105-3Flag BUB1-13myc (SBY9226), SPC105-3FLAG BUB1-13myc pGAL-MPS1 (SBY10100). (F) GLC7 (SBY9337) and pGAL-GLC7 (SBY10479) cells containing BUB1-3GFP and MTW1-mCherry were induced with or without galactose for 1 hour. The percentage of cells with Bub1-3GFP foci localizing to the kinetochore was quantified by microscopy. Scale bar = 5.0 microns.
Figure 4. The conserved MELT motifs in Spc105 regulate Bub1 localization to kinetochores and the spindle checkpoint
(A) A diagram of the N-terminal region of Spc105 shows the location of 6 MELT-like motifs illustrated as green bars. Residues that were detected as phosphorylated after Mps1 treatment are indicated with an asterisk. The N-terminus of Spc105 is denoted by “N” and the middle domain of the protein by “M”. Table shows MELT-like motifs (bolded) and surrounding sequence. (B) Kinetochores were purified (silver stain, right) from strains containing
SPC105-9myc (SBY10267) or spc105-6A-9myc (SBY10265). They were incubated with radioactive ATP and the corresponding phosphate incorporation was quantified (autorad, left). Standard deviation of normalized Spc105 signal is +/− 5% (n=3). (C) Bub1-GFP was immunoprecipitated from strains containing Spc105-9myc (SBY10266) or Spc105-6A-9myc (SBY10264). (D) Dsn1-Flag was immunoprecipitated from strains containing SPC105-9myc Bub1-3GFP (SBY10426) or Spc105-6A-9myc Bub1-3GFP (SBY10422). (E) Kinetochore particles were purified from strains containing Bub1-3GFP and SPC105 (SBY10426) or spc105-6A (SBY10422). The particles were treated with PP1 and then incubated with +/− ATP. They were then incubated in lysates from WT strains (SBY10426) containing Bub1-3GFP and analyzed by immunoblotting. (F) Strains containing BUB1-GFP MTW1-mCherry and SPC105-9myc (SBY10266) or spc105-6A-9myc (SBY10264) were arrested in nocodazole for 60 minutes. The percentage of cells showing co-localization of Bub1 with Mtw1 was quantified. Note that Bub1 is enriched on kinetochores that have detached from microtubules and are no longer clustered together . (G) Five-fold serial dilutions of WT (SBY3), SPC105-9myc (SBY10267), mad2Δ(SBY292), Ubi-R-TetR-BUB1 (SBY8920), or two independently generated spc105-6A-9myc strains (SBY10265, SBY10254) were analyzed for growth on YPD with doxycycline in the presence or absence of 10 µg/ml benomyl. (H) Pds1 levels were analyzed in SPC105-6His (SBY10373) or spc105-6A-6His (SBY10374) cells released from G1 into nocodazole. Pgk1 is a loading control. (H) Model for silencing of the spindle checkpoint. When kinetochores are not correctly attached to microtubules, Mps1 phosphorylates Spc105 to recruit Bub1/3. Proper microtubule attachment promotes PP1 interaction with Spc105, leading to dephosphorylation of Spc105 and release of Bub1/3 to promote checkpoint silencing.
Dual Mechanisms Regulate the Recruitment of Spindle Assembly Checkpoint Proteins to the Budding Yeast Kinetochore
P Aravamudhan et al.
Mol Biol Cell 27 (22), 3405-3417.
Recruitment of spindle assembly checkpoint (SAC) proteins by an unattached kinetochore leads to SAC activation. This recruitment is licensed by the Mps1 kinase, which pho …
KNL1/Spc105 Recruits PP1 to Silence the Spindle Assembly Checkpoint
JS Rosenberg et al.
Curr Biol 21 (11), 942-7.
The spindle assembly checkpoint (SAC) delays anaphase onset until kinetochores accomplish bioriented microtubule attachments . Although several centromeric and kinetoc …
Phosphodependent Recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 Kinase Maintains the Spindle Checkpoint
LA Shepperd et al.
Curr Biol 22 (10), 891-9.
The spindle assembly checkpoint (SAC) is the major surveillance system that ensures that sister chromatids do not separate until all chromosomes are correctly bioriented …
Spindle Checkpoint Silencing: PP1 Tips the Balance
B Lesage et al.
Curr Biol 21 (21), R898-903.
The spindle checkpoint is a mitotic surveillance mechanism that delays anaphase until all sister chromatids are correctly attached to microtubules from opposite poles. Re …
The Composition, Functions, and Regulation of the Budding Yeast Kinetochore
Genetics 194 (4), 817-46.
The propagation of all organisms depends on the accurate and orderly segregation of chromosomes in mitosis and meiosis. Budding yeast has long served as an outstanding mo …
PubMed Central articles
The Opposing Functions of Protein Kinases and Phosphatases in Chromosome Bipolar Attachment
D Sherwin et al.
Int J Mol Sci 20 (24).
Accurate chromosome segregation during cell division is essential to maintain genome integrity in all eukaryotic cells, and chromosome missegregation leads to aneuploidy …
Histone H2A Phosphorylation Recruits Topoisomerase IIα to Centromeres to Safeguard Genomic Stability
M Zhang et al.
EMBO J 39 (3), e101863.
Chromosome segregation in mitosis requires the removal of catenation between sister chromatids. Timely decatenation of sister DNAs at mitotic centromeres by topoisomerase …
Delineating the Contribution of Spc105-bound PP1 to Spindle Checkpoint Silencing and Kinetochore Microtubule Attachment Regulation
B Roy et al.
J Cell Biol 218 (12), 3926-3942.
Accurate chromosome segregation during cell division requires the spindle assembly checkpoint (SAC), which detects unattached kinetochores, and an error correction mechan …
Enrichment of Aurora B Kinase at the Inner Kinetochore Controls Outer Kinetochore Assembly
MK Bonner et al.
J Cell Biol 218 (10), 3237-3257.
Outer kinetochore assembly enables chromosome attachment to microtubules and spindle assembly checkpoint (SAC) signaling in mitosis. Aurora B kinase controls kinetochore …
PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore
RJ Smith et al.
Cell Rep 28 (8), 2206-2219.e8.
PP1 and PP2A-B56 are major serine/threonine phosphatase families that achieve specificity by colocalizing with substrates. At the kinetochore, however, both phosphatases …
Research Support, American Recovery and Reinvestment Act
Research Support, N.I.H., Extramural
Gene Expression Regulation, Fungal
Kinetochores / metabolism
M Phase Cell Cycle Checkpoints / physiology
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism
Phosphorylation / physiology
Protein Phosphatase 1 / genetics
Protein Phosphatase 1 / metabolism
Protein-Serine-Threonine Kinases / genetics
Protein-Serine-Threonine Kinases / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism
Saccharomyces cerevisiae Proteins
Spc105 protein, S cerevisiae
Bub1 spindle checkpoint protein
MPS1 protein, S cerevisiae
LinkOut - more resources
Full Text Sources Other Literature Sources Molecular Biology Databases