CXCR7: a novel tumor endothelial marker in renal cell carcinoma

Pathol Int. 2012 May;62(5):309-17. doi: 10.1111/j.1440-1827.2012.02792.x. Epub 2012 Feb 21.


Tumor angiogenesis is necessary for progression and metastasis of solid tumor. Tumor blood vessels are morphologically different from their normal counterparts. In this study, we isolated tumor endothelial cells (TECs) and revealed their abnormalities. We have compared the gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and found that several genes were upregulated in TECs. Expression of the chemokine receptor CXCR7 mRNA was higher in TECs than in NECs. However, information regarding the expression of CXCR7 in the tumor vessels of renal cell carcinoma is limited. CXCR7 and its ligand CXCL12 have been implicated in tumor cell survival. In this study, the expression of CXCR7 in the tumor vessels of renal cell carcinoma (RCC) was investigated. Real-time PCR revealed higher expression level of CXCR7 in cultured TECs than in cultured NECs. Furthermore, similar to mouse TECs, immunostaining revealed strong expression of CXCR7 in vivo in human tumor vessels. These findings suggest that CXCR7 is a novel TEC marker and a target for antiangiogenic therapy for RCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Biomarkers, Tumor / genetics*
  • Carcinoma, Renal Cell / blood supply
  • Carcinoma, Renal Cell / genetics*
  • Carcinoma, Renal Cell / pathology
  • Cell Line, Tumor
  • Chemokine CXCL12 / genetics
  • Chemokine CXCL12 / metabolism
  • Endothelium, Vascular / metabolism
  • Endothelium, Vascular / pathology*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Kidney Neoplasms / blood supply
  • Kidney Neoplasms / genetics*
  • Kidney Neoplasms / pathology
  • Male
  • Mice
  • Mice, Nude
  • Middle Aged
  • Neovascularization, Pathologic / metabolism
  • Neovascularization, Pathologic / pathology*
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / analysis
  • Receptors, CXCR / genetics*
  • Receptors, CXCR / metabolism
  • Up-Regulation


  • ACKR3 protein, human
  • Biomarkers, Tumor
  • CXCL12 protein, human
  • Chemokine CXCL12
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, CXCR