A set of GFP organelle marker lines for intracellular localization studies in Medicago truncatula

Plant Sci. 2012 Jun;188-189:19-24. doi: 10.1016/j.plantsci.2012.02.006. Epub 2012 Feb 17.

Abstract

Genomics advances in the model legume, Medicago truncatula, have led to an increase in the number of identified genes encoding proteins with unknown biological function. Determining the intracellular location of uncharacterized proteins often aids in the elucidation of biological function. To expedite such localization studies, we have generated a set of intracellular organelle green fluorescence protein (GFP) marker lines in M. truncatula. In addition to fluorescent detection, this set of organelle marker lines can also be used in immunohistochemical and cellular fractionation detection assays. Moreover, this set of marker lines is compatible with both transient and stable expression systems. Thus, this marker set should prove to be a useful resource for the M. truncatula research community.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers
  • Fluorescence
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Luminescent Agents / metabolism*
  • Medicago truncatula / genetics
  • Medicago truncatula / metabolism*
  • Medicago truncatula / ultrastructure
  • Organelles / metabolism*
  • Organelles / ultrastructure
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Plasmids
  • Protein Transport

Substances

  • Biomarkers
  • Luminescent Agents
  • Plant Proteins
  • Green Fluorescent Proteins