Key cytomembrane ABC transporters of Saccharomyces cerevisiae fail to improve the tolerance to D-limonene

Biotechnol Lett. 2012 Aug;34(8):1505-9. doi: 10.1007/s10529-012-0931-6. Epub 2012 Apr 24.


ATP-binding cassette transporters (ABC) are important detoxification proteins and were proposed to play important roles in monoterpene resistance in Saccharomyces cerevisiae. In this work, the transcriptional levels of typical ABC transporters of S. cerevisiae under 85 mg D-limonene/l were evaluated using real-time quantitative PCR. Only the transcriptional level of PDR5, YOR1 and PDR15 were upregulated but overexpression of these genes in S. cerevisiae failed to improve D-limonene tolerance suggesting that other mechanisms are involved in tolerance of yeast to monoterpenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism*
  • Cyclohexenes / chemistry
  • Cyclohexenes / pharmacology*
  • Drug Resistance, Fungal
  • Gene Expression Regulation, Fungal / drug effects
  • Limonene
  • Real-Time Polymerase Chain Reaction
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Terpenes / chemistry
  • Terpenes / pharmacology*


  • ATP-Binding Cassette Transporters
  • Cyclohexenes
  • Saccharomyces cerevisiae Proteins
  • Terpenes
  • Limonene