Abstract
Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.
MeSH terms
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Adult
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Antibodies, Viral / blood
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Brain / ultrastructure
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Brain / virology
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Cuba
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DNA, Viral / analysis
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Dengue Virus / genetics*
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Dengue Virus / isolation & purification
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Dengue Virus / ultrastructure*
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Enzyme-Linked Immunosorbent Assay
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Fatal Outcome
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Female
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Heart / virology
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Humans
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Immunoglobulin M / blood
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Kidney / ultrastructure
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Kidney / virology
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Liver / ultrastructure
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Liver / virology
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Microscopy, Electron, Transmission / methods
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Reverse Transcriptase Polymerase Chain Reaction
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Severe Dengue / diagnosis*
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Severe Dengue / virology
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Spleen / ultrastructure
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Spleen / virology
Substances
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Antibodies, Viral
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DNA, Viral
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Immunoglobulin M