Ragweed IgE and IgG4 antibody in nasal secretions during immunotherapy

Clin Exp Allergy. 1990 Sep;20(5):571-9. doi: 10.1111/j.1365-2222.1990.tb03152.x.

Abstract

We have developed sensitive amplified immunoassays for measurement of IgE and IgG4 ragweed (RW) antibodies in unconcentrated nasal washes. IgE to Amb a I (formerly antigen E) can be assayed to less than 0.1 ng/ml using IgE capture by anti-IgE on microtitre plates and an alkaline phosphatase-conjugated Amb a I with an amplification substrate technique. IgG4 to whole RW extract was assayed to less than 0.01 ng/ml by amplification ELISA using monoclonal anti-IgG4. Nasal washes (NW) (10 ml) and serum were obtained in December from 22 RW-sensitive patients before and after 1 and 2 yr of RW immunotherapy (IT), and assayed for Amb a I IgE or RW IgE and RW IgG4 antibodies Amb a I IgE could be measured in the NW of 15/22 pre IT, 19/22 at 1 yr IT, but only 3/10 at 2 yr IT (compared with pre-IT, P less than 0.05). Mean Amb a I IgE in NW was 0.66, 0.36 and 0.21 ng/ml at pre, 1 yr and 2 yr IT (P-values greater than 0.05). Mean serum RW IgE, was 76, 55 and 27 ng/ml at pre, 1 yr and 2 yr IT (P-values greater than 0.05). Amb a I IgE in nasal washes was correlated with RW IgE in serum (r = 0.56, P less than 0.001, n = 44). RW IgG4 was detectable in NW of 15/22 pre-IT, 18/22 at 1 yr IT and 9/10 at 2 yr IT (P-values greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Allergens*
  • Antigens, Plant
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin E / analysis*
  • Immunoglobulin G / analysis*
  • Immunotherapy*
  • Nasal Mucosa / chemistry*
  • Plant Proteins*
  • Pollen / analysis
  • Pollen / immunology*
  • Rhinitis, Allergic, Seasonal / immunology*
  • Rhinitis, Allergic, Seasonal / therapy

Substances

  • Allergens
  • Amb a I protein, Ambrosia artemisiifolia
  • Antigens, Plant
  • Immunoglobulin G
  • Plant Proteins
  • Immunoglobulin E