Visualization of candidate division OP3 cocci in limonene-degrading methanogenic cultures

J Microbiol Biotechnol. 2012 Apr;22(4):457-61. doi: 10.4014/jmb.1110.10055.


Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / metabolism*
  • Cyclohexenes / metabolism*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Limonene
  • Methane / metabolism*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Terpenes / metabolism*


  • Cyclohexenes
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Terpenes
  • Limonene
  • Methane