The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors

Gene. 1990 Oct 30;95(1):91-8. doi: 10.1016/0378-1119(90)90418-q.

Abstract

We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carboxy-Lyases / metabolism*
  • Chromosome Deletion
  • Escherichia coli / genetics
  • Genes, Fungal*
  • Genetic Linkage
  • Genetic Vectors
  • Molecular Sequence Data
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • Carboxy-Lyases
  • phosphoribosylaminoimidazole carboxylase

Associated data

  • GENBANK/M32013
  • GENBANK/M59824