[Inhibitory effects of sporoderm-broken Ganoderma lucidum spores on growth of lymphoma implanted in nude mouse]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Apr;20(2):310-4.
[Article in Chinese]

Abstract

This study was aimed to investigate the inhibitory effects of sporoderm-broken ganoderma lucidum spores (GLS) on transplanted lymphoma in nude mice and its mechanism. The models of the subcutaneously transplanting tumor were established by N-methylnitrosourea-induced thymus T-cell lymphoma. The cellular apoptosis of tumor tissues in nude mice were observed by transmission electron microscopy (TEM), the mRNA expression of Bax, Bcl-2, Bcl-xl was determined by RT-PCR. The results indicated that the GLS had a certain anti-tumor effect, and the inhibitory rate was 45.8 with the dose of 4 g/kg (ig, once daily for 21 d). Apoptotic cells in lymphoma cells treated with GLS were observed by TEM. RT-PCR analysis showed that the expression of Bax was up-regulated, Bcl-2 and Bcl-xl were down-regulated in T-cell lymphoma cells. It is concluded that GLS can inhibit proliferation of lymphoma cells and induce the lymphoma cell apoptosis, the mechanism of which may be related with up-regulating the expression of Bax and down-regulating the expression of Bcl-2 and Bcl-xl.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Apoptosis
  • Gene Expression Regulation, Neoplastic
  • Lymphoma, T-Cell / metabolism*
  • Lymphoma, T-Cell / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Nude
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2
  • Reishi*
  • Spores, Fungal*
  • Thymus Neoplasms / metabolism*
  • Thymus Neoplasms / prevention & control
  • Transplantation, Heterologous
  • bcl-2-Associated X Protein / metabolism
  • bcl-X Protein / metabolism

Substances

  • Bax protein, mouse
  • Bcl2l1 protein, mouse
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • bcl-X Protein
  • Bcl2 protein, mouse