Mimicking normal tissue architecture and perturbation in cancer with engineered micro-epidermis

Biomaterials. 2012 Jul;33(21):5221-9. doi: 10.1016/j.biomaterials.2012.04.009. Epub 2012 Apr 26.


Correct tissue architecture is essential for normal physiology, yet there have been few attempts to recreate tissues using micro-patterning. We have used polymer brush micro-engineering to generate a stratified micro-epidermis with fewer than 10 human keratinocytes. Epidermal stem cells are captured on 100 μm diameter circular collagen-coated disks. Within 24 h they assemble a stratified micro-tissue, in which differentiated cells have a central suprabasal location. For rings with a non-adhesive centre of up to 40 μm diameter, cell-cell and cell-matrix adhesive interactions together result in correct micro-epidermis assembly. Assembly requires actin polymerization, adherens junctions and desmosomes, but not myosin II-mediated contractility nor coordinated cell movement. Squamous cell carcinoma cells on micro-patterned rings exhibit disturbed architecture that correlates with the characteristics of the original tumours. The micro-epidermis we have generated provides a new platform for screening drugs that modulate tissue assembly, quantifying tissue stratification and investigating the properties of tumour cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adherens Junctions / metabolism
  • Animals
  • Cadherins / metabolism
  • Cell Line, Tumor
  • Cytoskeleton / metabolism
  • Desmosomes / metabolism
  • Epidermis / physiology*
  • Humans
  • Mice
  • Neoplasms / pathology*
  • Skin, Artificial*
  • Tissue Engineering / methods*


  • Cadherins