A novel bridge between oxidative stress and immunity: the interaction between hydrogen peroxide and human leukocyte antigen G in placental trophoblasts during preeclampsia

Am J Obstet Gynecol. 2012 May;206(5):447.e7-16. doi: 10.1016/j.ajog.2012.03.013. Epub 2012 Mar 20.


Objective: The aim of this study was to investigate a possible association between hydrogen peroxide and human leukocyte antigen G (HLA-G) in preeclampsia.

Study design: We explored the correlation between HLA-G and hydrogen peroxide in preeclamptic (n = 30) and normotensive (n = 30) placentas, which was confirmed by in vitro experiments. Furthermore, RNA interference was used to explore the influence of HLA-G on the proliferation, apoptosis and invasion of HTR-8/SVneo cells when exposed to hydrogen peroxide.

Results: We found an inverse correlation between hydrogen peroxide and HLA-G expression in preeclamptic placentas. High levels of hydrogen peroxide could down-regulate HLA-G expression in HTR-8/SVneo. Compared with HLA-G knockdown HTR-8/SVneo, increased proliferation inhibition, higher apoptosis, and decreased cell invasion were found in the cell expressing HLA-G when exposed to hydrogen peroxide.

Conclusion: Our findings highlight that high levels of hydrogen peroxide can down-regulate HLA-G expression in trophoblasts during preeclampsia and trophoblasts expressing HLA-G are vulnerable to oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apoptosis
  • Biomarkers / metabolism
  • Blotting, Western
  • Case-Control Studies
  • Cell Line
  • Cell Survival
  • Down-Regulation
  • Female
  • Fluorescent Antibody Technique
  • HLA-G Antigens / metabolism*
  • Humans
  • Hydrogen Peroxide / metabolism*
  • Oxidative Stress*
  • Placenta / metabolism*
  • Pre-Eclampsia / etiology*
  • Pre-Eclampsia / immunology
  • Pregnancy
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trophoblasts / metabolism
  • Trophoblasts / physiology


  • Biomarkers
  • HLA-G Antigens
  • Hydrogen Peroxide