Antigenotoxic effect of acute, subacute and chronic treatments with Amazonian camu-camu (Myrciaria dubia) juice on mice blood cells

Food Chem Toxicol. 2012 Jul;50(7):2275-81. doi: 10.1016/j.fct.2012.04.021. Epub 2012 Apr 21.


Myrciaria dubia, a plant native to the Amazon region, stands out as a fruit rich in vitamin C and other metabolites with nutritional potential. We evaluated the antioxidant, genotoxic and antigenotoxic potential of M. dubia juice on blood cells of mice after acute, subacute and chronic treatments. Flavonoids and vitamin C present in the fruit of M. dubia were quantified. In vitro antioxidant activity was evaluated by DPPH assay. Blood samples were collected for analysis after treatment, and the alkaline comet assay was used to analyze the genotoxic and antigenotoxic activity (ex vivo analysis using H(2)O(2)). The amount of vitamin C per 100mL of M. dubia was 52.5mg. DPPH assay showed an antioxidant potential of the fruit. No M. dubia concentration tested exerted any genotoxic effect on mice blood cells. In the ex vivo test, the juice demonstrated antigenotoxic effect, and acute treatment produced the most significant results. After the treatments, there was no evidence of toxicity or death. In conclusion, our data show that M. dubia juice has antigenotoxic and antioxidant activities, though with no genotoxicity for blood cells. Nevertheless, more in-depth studies should be conducted to assess the safety of this fruit for human consumption.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimutagenic Agents / pharmacology*
  • Antimutagenic Agents / toxicity
  • Blood Cells / drug effects*
  • Comet Assay
  • Female
  • Male
  • Mice
  • Mutagens / toxicity
  • Myrtaceae / chemistry*
  • Plant Extracts / pharmacology*
  • Toxicity Tests, Acute
  • Toxicity Tests, Chronic
  • Toxicity Tests, Subacute


  • Antimutagenic Agents
  • Mutagens
  • Plant Extracts