Genome-wide association mapping of flowering time and northern corn leaf blight (Setosphaeria turcica) resistance in a vast commercial maize germplasm set

BMC Plant Biol. 2012 Apr 30;12:56. doi: 10.1186/1471-2229-12-56.

Abstract

Background: Setosphaeria turcica is a fungal pathogen that causes northern corn leaf blight (NCLB) which is a serious foliar disease in maize. In order to unravel the genetic architecture of the resistance against this disease, a vast association mapping panel comprising 1487 European maize inbred lines was used to (i) identify chromosomal regions affecting flowering time (FT) and northern corn leaf blight (NCLB) resistance, (ii) examine the epistatic interactions of the identified chromosomal regions with the genetic background on an individual molecular marker basis, and (iii) dissect the correlation between NCLB resistance and FT.

Results: The single marker analyses performed for 8 244 single nucleotide polymorphism (SNP) markers revealed seven, four, and four SNP markers significantly (α=0.05, amplicon wise Bonferroni correction) associated with FT, NCLB, and NCLB resistance corrected for FT, respectively. These markers explained individually between 0.36 and 14.29% of the genetic variance of the corresponding trait.

Conclusions: The very well interpretable pattern of SNP associations observed for FT suggested that data from applied plant breeding programs can be used to dissect polygenic traits. This in turn indicates that the associations identified for NCLB resistance might be successfully used in marker-assisted selection programs. Furthermore, the associated genes are also of interest for further research concerning the mechanism of resistance to NCLB and plant diseases in general, because some of the associated genes have not been mentioned in this context so far.

MeSH terms

  • Ascomycota / physiology
  • Chromosome Mapping*
  • Disease Resistance / immunology*
  • Epistasis, Genetic
  • Flowers / genetics*
  • Flowers / physiology
  • Genome-Wide Association Study*
  • Genotype
  • Inbreeding
  • Phenotype
  • Plant Diseases / genetics
  • Plant Diseases / immunology
  • Plant Diseases / microbiology*
  • Plant Leaves / genetics
  • Plant Leaves / immunology
  • Plant Leaves / microbiology*
  • Polymorphism, Single Nucleotide / genetics
  • Regression Analysis
  • Seeds / genetics
  • Seeds / immunology
  • Seeds / microbiology
  • Time Factors
  • Zea mays / genetics*
  • Zea mays / immunology
  • Zea mays / microbiology