Abstract
Multidrug-resistant clinical isolate Klebsiella pneumoniae BM4686 was highly resistant to 4,6-disubstituted 2-deoxystreptamines and to fortimicin. Resistance was due to the presence, on the 40-kb non-self-transferable plasmid pIP849, of the rmtF gene which was cotranscribed with the upstream aac(6')-Ib gene. The deduced RmtF protein had 25 to 46% identity with members of the N7 G1405 family of aminoglycoside resistance 16S rRNA methyltransferases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoglycosides / pharmacology*
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics
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Methyltransferases / genetics
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Methyltransferases / metabolism*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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RNA, Ribosomal, 16S / metabolism*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Bacterial Proteins
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RNA, Ribosomal, 16S
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fortimicins
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Methyltransferases