Characterization of a Chitinase (Chit62) From Serratia Marcescens B4A and Its Efficacy as a Bioshield Against Plant Fungal Pathogens

Biochem Genet. 2012 Oct;50(9-10):722-35. doi: 10.1007/s10528-012-9515-3. Epub 2012 May 4.

Abstract

Chitinases have been suggested to be involved in pathogen-antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters. Active enzyme was used to determine its biocontrol features against fungal phytopathogens. The results demonstrated that the optimum pH and temperature for the enzyme activity were 6.0 and 55 °C, respectively. The K(m) and V(max) values were 3.30 mg ml(-1) and 0.92 units, respectively. The recombinant chitinase was demonstrated to be highly active in controlling fungal pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternaria / drug effects
  • Alternaria / growth & development
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification*
  • Bacterial Proteins / pharmacology
  • Biological Products / isolation & purification
  • Biological Products / pharmacology
  • Chitin / metabolism
  • Chitinases / genetics
  • Chitinases / isolation & purification*
  • Chitinases / pharmacology
  • Computational Biology
  • DNA, Bacterial
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Activation
  • Enzyme Assays
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fusarium / drug effects
  • Fusarium / growth & development
  • Hydrogen-Ion Concentration
  • Microbial Sensitivity Tests
  • Mycelium / drug effects
  • Mycelium / growth & development
  • Phylogeny
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology
  • Rhizoctonia / drug effects
  • Rhizoctonia / growth & development
  • Serratia marcescens / enzymology*
  • Serratia marcescens / genetics
  • Temperature

Substances

  • Antifungal Agents
  • Bacterial Proteins
  • Biological Products
  • DNA, Bacterial
  • Recombinant Proteins
  • Chitin
  • Chitinases