MicroRNA-19a and -19b regulate cervical carcinoma cell proliferation and invasion by targeting CUL5

Cancer Lett. 2012 Sep 28;322(2):148-58. doi: 10.1016/j.canlet.2012.02.038. Epub 2012 May 2.

Abstract

MicroRNAs (miRNAs) play a key role in the regulation of gene expression. In this study, we demonstrate that microRNA-19a and -19b (miR-19a/b) are highly expressed in human cervical cancer cells and are involved in maintaining the malignant phenotypes of HeLa and C33A cells. Up-regulation of miR-19a and miR-19b promoted cell growth and invasion, whereas knockdown of miR-19a and miR-19b yielded the reverse phenotype. CUL5 was identified as a novel target gene of both miR-19a and miR-19b. CUL5 ectopic over-expression without its 3' untranslated region (UTR) abolished the effect of miR-19a/b on HeLa and C33A cell proliferation and invasion. These results indicated that miR-19a/b directly and negatively regulate CUL5 expression in cervical cancer cells, highlighting the importance of miR-19a and miR-19b and their target genes in tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Base Sequence
  • Carcinoma / genetics*
  • Carcinoma / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Transformation, Neoplastic / genetics
  • Cullin Proteins / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Silencing
  • HeLa Cells
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Neoplasm Invasiveness / genetics
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / metabolism

Substances

  • 3' Untranslated Regions
  • CUL5 protein, human
  • Cullin Proteins
  • MIRN19 microRNA, human
  • MicroRNAs