Abstract
Analysis of global temporal gene expression by human intestinal cells when exposed to Lactobacillus acidophilus revealed induction of immune-related pathways and NF-κB target genes after a 1-h exposure, compared to a 4- or 8-h exposure. Additionally, an L. acidophilus derivative expressing covalently bound flagellin resulted in increased induction of il8, cxc1, and cxcl2 compared to the parent L. acidophilus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Caco-2 Cells
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Epithelial Cells / immunology
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Epithelial Cells / metabolism
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Epithelial Cells / microbiology*
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Flagellin / metabolism
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Flagellin / pharmacology
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Gene Expression Profiling*
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Gene Expression Regulation*
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Humans
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Intestinal Mucosa / metabolism
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Intestines / cytology
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Intestines / immunology
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Intestines / microbiology*
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Lactobacillus acidophilus / immunology*
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Molecular Sequence Data
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Probiotics / pharmacology*
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Time Factors
Substances
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NF-kappa B
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Recombinant Proteins
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Flagellin