Influence of exposure time on gene expression by human intestinal epithelial cells exposed to Lactobacillus acidophilus

Appl Environ Microbiol. 2012 Jul;78(14):5028-32. doi: 10.1128/AEM.00504-12. Epub 2012 May 4.

Abstract

Analysis of global temporal gene expression by human intestinal cells when exposed to Lactobacillus acidophilus revealed induction of immune-related pathways and NF-κB target genes after a 1-h exposure, compared to a 4- or 8-h exposure. Additionally, an L. acidophilus derivative expressing covalently bound flagellin resulted in increased induction of il8, cxc1, and cxcl2 compared to the parent L. acidophilus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caco-2 Cells
  • Epithelial Cells / immunology
  • Epithelial Cells / metabolism
  • Epithelial Cells / microbiology*
  • Flagellin / metabolism
  • Flagellin / pharmacology
  • Gene Expression Profiling*
  • Gene Expression Regulation*
  • Humans
  • Intestinal Mucosa / metabolism
  • Intestines / cytology
  • Intestines / immunology
  • Intestines / microbiology*
  • Lactobacillus acidophilus / immunology*
  • Molecular Sequence Data
  • NF-kappa B / genetics
  • NF-kappa B / metabolism
  • Probiotics / pharmacology*
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Time Factors

Substances

  • NF-kappa B
  • Recombinant Proteins
  • Flagellin

Associated data

  • GEO/GSE34175