Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1

Arch Microbiol. 1990;154(5):489-95. doi: 10.1007/BF00245233.

Abstract

Growth of Klebsiella pneumoniae M5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumaryl-pyruvate hydrolase. Growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. Mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normally on 2,5-dihydroxybenzoate. Mutants lacking maleylpyruvate isomerase fail to grow on 3-hydroxybenzoate and 2,5-dihydroxybenzoate. Both kinds of mutants grow normally on 3,4-dihydroxybenzoate. Mutants defective in maleylpyruvate isomerase accumulate maleylpyruvate when exposed to 3-hydroxybenzoate and growth is inhibited. Secondary mutants that have additionally lost 3-hydroxybenzoate monooxygenase are no longer inhibited by the presence of 3-hydroxybenzoate. The 3-hydroxybenzoate monooxygenase gene (mhbM) and the maleylpyruvate isomerase gene (mhbI) are 100% co-transducible by P1 phage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Ion Exchange
  • Gentisates / metabolism*
  • Hydroxybenzoates / metabolism*
  • Isomerases / genetics
  • Isomerases / metabolism
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / growth & development
  • Klebsiella pneumoniae / metabolism*
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism
  • Mutation
  • Oxidation-Reduction
  • Transduction, Genetic
  • cis-trans-Isomerases*

Substances

  • Gentisates
  • Hydroxybenzoates
  • 3-hydroxybenzoic acid
  • protocatechuic acid
  • Mixed Function Oxygenases
  • 3-hydroxybenzoate-4-monooxygenase
  • Isomerases
  • cis-trans-Isomerases
  • maleylpyruvate isomerase
  • 2,5-dihydroxybenzoic acid