Rendering protein-based particles transiently insoluble for therapeutic applications

J Am Chem Soc. 2012 May 30;134(21):8774-7. doi: 10.1021/ja302363r. Epub 2012 May 17.


Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker leaves no chemical residue on the reactive amino group. Delivery of a self-replicating RNA was achieved via the transiently insoluble PRINT protein particles. These protein particles can provide new opportunities for drug and gene delivery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chlorocebus aethiops
  • Cytoplasm / metabolism
  • Disulfides / chemistry
  • Drug Carriers / chemistry*
  • Drug Carriers / metabolism
  • Microtechnology / methods*
  • Nanotechnology / methods*
  • Particle Size
  • RNA / metabolism
  • Serum Albumin, Bovine / chemistry*
  • Serum Albumin, Bovine / metabolism
  • Solubility
  • Time Factors
  • Vero Cells


  • Disulfides
  • Drug Carriers
  • Serum Albumin, Bovine
  • RNA