Enhanced antiviral activity of acyclovir loaded into nanoparticles

Methods Enzymol. 2012:509:1-19. doi: 10.1016/B978-0-12-391858-1.00001-0.

Abstract

The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a β-cyclodextrin-poly(4-acryloylmorpholine) monoconjugate (β-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be described in this chapter. Fluorescent labeled nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry*
  • Acyclovir / metabolism
  • Acyclovir / pharmacology*
  • Animals
  • Antiviral Agents / metabolism
  • Antiviral Agents / pharmacology*
  • Cell Survival / drug effects
  • Chlorocebus aethiops
  • Complement Activation / drug effects
  • Hemolysis / drug effects
  • Herpesvirus 1, Human / drug effects*
  • Herpesvirus 1, Human / growth & development
  • Humans
  • Materials Testing
  • Nanocapsules / chemistry*
  • Particle Size
  • Reactive Oxygen Species / metabolism
  • Skin / drug effects
  • Surface Properties
  • Tissue Culture Techniques
  • Vero Cells
  • Viral Load
  • Viral Plaque Assay
  • beta-Cyclodextrins / chemistry*

Substances

  • Acrylic Resins
  • Antiviral Agents
  • Nanocapsules
  • Reactive Oxygen Species
  • beta-Cyclodextrins
  • poly(acryloylmorpholine)
  • Acyclovir