PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Biochem Biophys Res Commun. 2012 Jun 1;422(2):250-5. doi: 10.1016/j.bbrc.2012.04.136. Epub 2012 Apr 30.


Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factor 6
  • ADP-Ribosylation Factors / metabolism
  • Actins / metabolism
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cytoskeleton / metabolism
  • Dendrites / metabolism
  • Dendrites / physiology*
  • Female
  • Gene Knockdown Techniques
  • Guanine Nucleotide Exchange Factors / metabolism
  • HEK293 Cells
  • Hippocampus / cytology*
  • Humans
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Neurites / metabolism
  • Neurites / physiology
  • Neurons / metabolism
  • Neurons / physiology*
  • Neurons / ultrastructure
  • PDZ Domains*
  • Rats
  • Rats, Sprague-Dawley


  • ADP-Ribosylation Factor 6
  • Actins
  • Carrier Proteins
  • GRASP protein, rat
  • Guanine Nucleotide Exchange Factors
  • Membrane Proteins
  • Psd protein, rat
  • ADP-Ribosylation Factors
  • ARF6 protein, human
  • Arf6 protein, rat