Cloning and expression of a cDNA for human cytochrome P-450aldo as related to primary aldosteronism

Biochem Biophys Res Commun. 1990 Nov 30;173(1):309-16. doi: 10.1016/s0006-291x(05)81058-7.


A cDNA clone encoding human aldosterone synthase cytochrome P-450 (P-450aldo) has been isolated from a cDNA library derived from human adrenal tumor of a patient suffering from primary aldosteronism. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 3 bp 5'-untranslated region and a 1424 bp 3'-untranslated region to which a poly(A) tract is attached. The nucleotide sequence of P-450aldo cDNA is 93% identical to that of P-450(11) beta cDNA. Catalytic functions of these two P-450s expressed in COS-7 cells are very similar in that both enzymes catalyze the formation of corticosterone and 18-hydroxy-11-deoxycorticosterone using 11-deoxycorticosterone as a substrate. However, they are distinctly different from each other in that P-450aldo preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone while P-450(11)beta substantially fails to catalyze the reaction to form aldosterone. These results suggest that P-450aldo is a variant of P-450(11)beta, but this enzyme is a different gene product possibly playing a major role in the synthesis of aldosterone at least in a patient suffering from primary aldosteronism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cytochrome P-450 CYP11B2
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • DNA / genetics*
  • Gene Expression*
  • Humans
  • Hyperaldosteronism / enzymology*
  • Hyperaldosteronism / genetics
  • Mitochondria / enzymology
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Steroid Hydroxylases / genetics*
  • Steroid Hydroxylases / metabolism
  • Substrate Specificity
  • Transfection


  • Oligonucleotide Probes
  • Recombinant Proteins
  • DNA
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Cytochrome P-450 CYP11B2

Associated data

  • GENBANK/X54741