Abstract
There is a close correlation between endothelin-1 (ET-1) and microRNA-1 (miRNA-1) expression in the heart, but whether ET-1 expression is regulated by miRNA-1 warrants further research. Our results revealed multiple clues suggesting that miRNA-1 may participate in inhibiting ET-1 gene expression. The inhibitory effect of miRNA-1 on recombinant luciferase reporter gene was mediated by the target sequence at the 127th nucleotide on ET-1 3'UTR. We further confirmed that miRNA-1 could inhibit endogenous ET-1 gene expression at the post-transcriptional level. Our study provides a new perspective on the regulatory mechanism of ET-1 gene.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Animals
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Binding Sites
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Cell Line
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Cells, Cultured
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Down-Regulation*
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Endothelin-1 / antagonists & inhibitors
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Endothelin-1 / genetics
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Endothelin-1 / metabolism*
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Genes, Reporter
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Human Umbilical Vein Endothelial Cells / metabolism
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Humans
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Mice
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MicroRNAs / genetics
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MicroRNAs / physiology*
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Models, Molecular
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Mutant Proteins / antagonists & inhibitors
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Mutant Proteins / metabolism
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Mutation
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Myoblasts / metabolism*
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Organ Specificity
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RNA, Messenger / metabolism
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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3' Untranslated Regions
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Endothelin-1
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MIRN1 microRNA, human
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MicroRNAs
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Mirn1 microRNA, mouse
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Mutant Proteins
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RNA, Messenger
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Recombinant Proteins