The inhibitory effect of miRNA-1 on ET-1 gene expression

FEBS Lett. 2012 Apr 5;586(7):1014-21. doi: 10.1016/j.febslet.2012.02.044. Epub 2012 Mar 15.

Abstract

There is a close correlation between endothelin-1 (ET-1) and microRNA-1 (miRNA-1) expression in the heart, but whether ET-1 expression is regulated by miRNA-1 warrants further research. Our results revealed multiple clues suggesting that miRNA-1 may participate in inhibiting ET-1 gene expression. The inhibitory effect of miRNA-1 on recombinant luciferase reporter gene was mediated by the target sequence at the 127th nucleotide on ET-1 3'UTR. We further confirmed that miRNA-1 could inhibit endogenous ET-1 gene expression at the post-transcriptional level. Our study provides a new perspective on the regulatory mechanism of ET-1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Binding Sites
  • Cell Line
  • Cells, Cultured
  • Down-Regulation*
  • Endothelin-1 / antagonists & inhibitors
  • Endothelin-1 / genetics
  • Endothelin-1 / metabolism*
  • Genes, Reporter
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • Mice
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Models, Molecular
  • Mutant Proteins / antagonists & inhibitors
  • Mutant Proteins / metabolism
  • Mutation
  • Myoblasts / metabolism*
  • Organ Specificity
  • RNA, Messenger / metabolism
  • Recombinant Proteins / antagonists & inhibitors
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • 3' Untranslated Regions
  • Endothelin-1
  • MIRN1 microRNA, human
  • MicroRNAs
  • Mirn1 microRNA, mouse
  • Mutant Proteins
  • RNA, Messenger
  • Recombinant Proteins